Summary
Type: SPE
Application #: 302400
Catalog#: 730011 - SPE Cartridges, Chromabond, C18 ec, 1ml, PP, 100mg, 100/pk
Detection: HPLC
Conditions: Column conditioning: (flow rate: 3,0 ml/min; air volume: 0,1 ml): At the beginning, the column holder is located above the drain cuvette (front position). The column is first washed with 2,0 ml of methanol, then with 2,0 ml of phosphate buffer pH 7,4 in order to prepare the sorbent to receive the plasma sample.
Sample application: (flow rate: 0,18 ml/min; air volume: 1,0 ml): The auto-sampler needle aspirates 1,0 ml of plasma from the corresponding vial and dispenses it on the column.
Column washing: (flow rate: 1,5 ml/min; air volume: 1,0 ml): 2,0 ml of phosphate buffer pH 7,4 are dispensed on the column. The packing is then flushed with 1,0 ml of air.
Elution: (flow rate: 1,5 ml/min; air volume: 1,0 ml): The column holder is pushed by the needle over the collection rack. A 0,25-ml volume of methanol is applied on the column. The eluate is collected in the tube positioned under the column
Dilution: (flow rate: 1,5 ml/min; air volume: 1,0 ml): 0,3 ml of phosphate buffer pH 7,4 are dispensed on the column. The column holder is then replaced in its position.
Authors: Hubert Ph Crommen J
Source: Journal of Liquid Chromatography 13(19) 3891-3907 (1990)